Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Serine protease inhibitor from the muscle larval Trichinella spiralis ameliorates non-alcoholic fatty liver disease in mice via anti-inflammatory properties and gut-liver crosstalk.

doi: 10.1016/j.biopha.2024.116223

Figure Lengend Snippet: Fig. 4. rTs-SPI attenuated macrophage infiltration and promoted CD4þCD25þFoxp3þ Tregs population in NAFLD mice. (A) Macrophage infiltration and (B) CD4+CD25+Foxp3+ Tregs in spleen using Flow cytometry analysis; Quantitative analysis of (C) F4/80+ macrophages and (D) CD4+CD25+Foxp3+ Tregs in spleen; (E) Microphotographs show total macrophages staining with F4/80 (red) and DAPI (blue) in liver (600x); (F) Quantitative analysis of F4/80+ macrophages in liver; The hepatic mRNA expression levels of (G) F4/80 and (H) Foxp3. Values are presented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: For Treg analysis, cells were stained with Percp/cy5.5 anti-mouse CD4 and APC anti-mouse CD25 antibodies (Elabscience, China), followed by fixing and permeabilizing using a Foxp3/transcription factor staining kit (Elabscience, China) and staining with PE anti-mouse Foxp3 antibody (Elabscience, China).

Techniques: Flow Cytometry, Staining, Expressing

Journal: Frontiers in Neuroscience

Article Title: The efficacy of an allosteric modulator of the alpha 7 nicotinic acetylcholine receptor in a murine model of stroke

doi: 10.3389/fnins.2025.1525975

Figure Lengend Snippet: PNU-120596 (PNU) administration does not alter the CD4 T-cell phenotype in the central nervous system (CNS)-relevant immune compartment. (A) Representative dot plot showing the CD4 gate on CD3 + events isolated from the cervical lymph nodes. In the bar graph, the left two columns indicate absolute counts of CD4 T-cells, while the right two columns indicate the relative percentages. There were no significant differences in the absolute count and percentages of CD4 T-cells between the vehicle and PNU-treated group. (B) Representative data showing the IL17 + gate on CD4 + events. Similarly, no changes were observed in the pathogenic Th17 (CD4 + IL-17A +) T-cells in either the absolute count or percentages. (C) Representative data showing the Foxp3 + gate on CD4 + events. The absolute count and percentages of regulatory (CD4 + Foxp3 + ) T-cells remained unchanged. (D) Representative data showing the IFN-γ + gate or IL4 + gate on CD4 + events. The pathogenic Th1 (CD4 + IFNy + ) and anti-inflammatory Th2 (CD4 + IL-4 + ) T-cells showed no significant differences in absolute values and percentages between the two groups. The Th1/Th2 ratio also showed no difference between the vehicle and PNU-treated group. The data represents three independent experiments, shown as the mean ± SD, and was analyzed using the Mann-Whitney U test; vehicle n = 6 mice, PNU n = 7 mice for all graphs except the Th1/Th2 ratio (vehicle n = 5 mice, PNU n = 7 mice).

Article Snippet: After incubation, the following antibodies were added: anti-mouse interferon gamma VioletFluor 450 (Cat. 75-7311-U100, Tonbo), anti-mouse Foxp3 PE-Cyanine 7 (Cat. 60-5773-U025, Tonbo), anti-mouse IL-17A FITC (Cat. 506907, BioLegend), and anti-mouse IL-4 PE (Cat. 50-7041-U025, Tonbo).

Techniques: Isolation, MANN-WHITNEY

Journal: Frontiers in Neuroscience

Article Title: The efficacy of an allosteric modulator of the alpha 7 nicotinic acetylcholine receptor in a murine model of stroke

doi: 10.3389/fnins.2025.1525975

Figure Lengend Snippet: PNU-120596 (PNU) administration does not alter the CD4 T-cell phenotype in the peripheral immune compartment. (A) Representative dot plot showing the CD4 gate on CD3 + events isolated from the spleen. There were no significant differences in the absolute count and percentages of CD4 T-cells between the vehicle and PNU-treated group. (B) No significant changes were observed in the pathogenic Th17 (CD4 + IL-17A + ) subsets in both the absolute count and percentage. (C) Regulatory T-cells (CD4 + Foxp3 + ) also exhibited no difference in either their absolute count or percentages. (D) The pathogenic Th1 (CD4 + INF-y + ) and anti-inflammatory Th2 (CD4 + IL-4+ + T-cells showed no significant difference in their absolute counts and percentages between the two cohorts. The Th1/Th2 ratio also showed no differences between the vehicle and PNU-treated group. The data represents three independent experiments, shown as the mean ± SD, and was analyzed using the Mann-Whitney U test; vehicle n = 9 mice, PNU n = 9 mice.

Article Snippet: After incubation, the following antibodies were added: anti-mouse interferon gamma VioletFluor 450 (Cat. 75-7311-U100, Tonbo), anti-mouse Foxp3 PE-Cyanine 7 (Cat. 60-5773-U025, Tonbo), anti-mouse IL-17A FITC (Cat. 506907, BioLegend), and anti-mouse IL-4 PE (Cat. 50-7041-U025, Tonbo).

Techniques: Isolation, MANN-WHITNEY

Journal: Cancer Cell International

Article Title: Dendritic cells matured with recombinant human sperm associated antigen 9 (rhSPAG9) induce CD4 + , CD8 + T cells and activate NK cells: a potential candidate molecule for immunotherapy in cervical cancer

doi: 10.1186/s12935-021-01951-7

Figure Lengend Snippet: FACS analysis of CD3 + /FoxP3 + Tregs in ( a ) Cisplatin untreated SPDCs stimulated co-cultures ( b ) Cisplatin treated (150 μM) SPDCs stimulated co-cultures ( c ) Bar graphs indicate CD3 + / FoxP3 + Tregs ratio in SPDCs stimulated co-cultures treated with three different doses of cisplatin treated SPDCs ( P = 0.340; P10–P12)

Article Snippet: The cells were further probed with anti-CD4-PC5, anti-CD8-APC, anti-CD56-PE, anti-CD25-ECD, anti-FOXP3-PE antibodies and Propidium iodide (1 µg/ml; Himedia, India) to verify the response of the autologous or allogeneic PBMCs upon stimulation with SPDCs.

Techniques: